Coding

Part:BBa_K1913013:Design

Designed by: Linea Katharina Muhsal   Group: iGEM16_Wageningen_UR   (2016-09-28)


Cry3Aa


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 1
    Illegal SpeI site found at 2048
    Illegal PstI site found at 2062
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal SpeI site found at 2048
    Illegal PstI site found at 2062
    Illegal NotI site found at 7
    Illegal NotI site found at 2055
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 1
    Illegal suffix found in sequence at 2048
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 1
    Illegal XbaI site found at 16
    Illegal SpeI site found at 2048
    Illegal PstI site found at 2062
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

4 forbidden restriction sites had to be removed


Source

Bacillus thuringiensis var. tenebrionis

References