Coding
Part:BBa_K1913013:Design
Designed by: Linea Katharina Muhsal Group: iGEM16_Wageningen_UR (2016-09-28)
Cry3Aa
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal prefix found in sequence at 1
Illegal SpeI site found at 2048
Illegal PstI site found at 2062 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
Illegal SpeI site found at 2048
Illegal PstI site found at 2062
Illegal NotI site found at 7
Illegal NotI site found at 2055 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
- 23INCOMPATIBLE WITH RFC[23]Illegal prefix found in sequence at 1
Illegal suffix found in sequence at 2048 - 25INCOMPATIBLE WITH RFC[25]Illegal prefix found in sequence at 1
Illegal XbaI site found at 16
Illegal SpeI site found at 2048
Illegal PstI site found at 2062 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
4 forbidden restriction sites had to be removed
Source
Bacillus thuringiensis var. tenebrionis